Novel Antimicrobial Peptides identified in Human Blood Ultrafiltrate
New, available since March 2012: Hepcidin (Rat) | Iron-Regulatory Peptide [13C18,15N3]-Hepcidin (Human) | Stable Isotope-Labeled Peptide for Mass Spectrometric Detection of Hepcidin (Human)
One methods aimed at finding peptides and proteins, is the systematic analysis of polypeptides in human blood ultrafiltrate, which has proven to be successful. Liver-expressed antimicrobial peptide 1 ( LEAP-1 / Hepcidin (Human) ) is an example of such, which elicits antimicrobial activity as well as some functions in iron homeostasis. After the description of LEAP-1 | Hepcidin, a new anti microbial peptide LEAP-2 has been recently discovered. [Protein Sci., 12, 143 (2003)]. The isolated peptides have variable chain length with the deletion of the longest peptide, composed of 40 amino acid residues, at both the amino- and carboxyl-termini. Its disulfide arrangement was determined to be Cys1-Cys3 and Cys2-Cys4 (Cys numbering from the amino- terminus) by analyzing the molecular weight and sequence of the tryptic fragments. The cDNA cloning of LEAP-2 reveals the precursor of LEAP-2 to be a 77-residue peptide, in which the longest form corresponds to the residues between the positions of 38 and 77 in the precursor [prepro- LEAP-2(38-77)]. The primary structures of LEAP-2 of rhesus monkey, cow, pig, mouse, and guinea pig were also determined, and found to be highly conserved among these species. LEAP-2 mRNA is predominantly expressed in liver and small intestine.
4405-s 0.1 mg Met - Thr - Pro - Phe - Trp - Arg - Gly - Val - Ser - Leu - Arg - Pro - Ile - Gly - Ala - Ser - Cys - Arg - Asp - Asp - Ser - Glu - Cys - Ile - Thr - Arg - Leu - Cys - Arg - Lys - Arg - Arg - Cys - Ser - Leu - Ser - Val - Ala - Gln - Glu
