Ghrelin (Rat)

4373-s 0.1 mg | 185.00 EUR

Gly – Ser – Ser(n- Octanoyl) – Phe – Leu – Ser – Pro – Glu – His – Gln – Lys – Ala – Gln – Gln – Arg – Lys – Glu – Ser – Lys – Lys – Pro – Pro – Ala – Lys – Leu – Gln – Pro – Arg

Synthetic Product (Trifluoracetate Form)

The purity is guaranteed to be higher than 99% by HPLC

Endogenous Growth-Hormone Releasing Peptide with Novel Regulatory Mechanism

Small synthetic molecules called growth-hormone secretagogues (GHSs) stimulate the release of growth hormone (GH) from pituitary through a G-protein-coupled receptor (GHS-R) [Science, 260, 1640 (1993); Cell. Mol. Life Sci., 54, 1316 (1998)]. However, the specific endogenous ligand for this receptor has long been unidentified, therefore, this receptor is considered to be one of the orphan receptors.

Endogenous ligand for GHS-R is now identified as ghrelin with the following characteristics:

1. ghrelin is a 28-residue peptide with a n-octanoyl group at Ser³,
2. ghrelin stimulates GH-release from rat primary cultured pituitary cells in a dose-dependent manner (EC₅₀ = 2.1 nM), and
3. ghrelin induces an increase of intracellular Ca²⁺ in GHS-R-expressing cells with EC₅₀ value of 2.5 nM [Nature, 402, 656(1999)].

Although ghrelin and the known hypothalamic peptide, growth-hormone releasing factor, stimulate GH-release, there is a distinction between these peptides both in GH secretion mechanism and in structural aspect. Octanoyl group attached on the side chain of Ser³ is essential for expressing activity. Rat and human peptide sequences are clarified to be mutually identical except for the amino acid substitutions at positions 11 and 12. Interestingly, the major ghrelin-producing tissue is the stomach, and ghrelin immunoreactivity is found in healthy human blood. This fact may provide the new regulation mechanism for GH-release; ghrelin secreted from the stomach circulates in the blood stream to the pituitary where this peptide exerts a GH-releasing function. It is conceivable that ghrelin may have other functions in some tissues other than pituitary, because GHS-R is expressed in heart, lung, pancreas, intestine and adipose tissue.

Shipping | Storage | Stability
Our peptide Ghrelin will be shipped at room temperature. Information about product specific storage conditions can be found on the vial. Stock solutions should be stored at -20°C. Please avoid repeated freezing and thawing by preparing aliquots.

References:

1. M. Kojima, H. Hosoda, Y. Date, M. Nakazato, H. Matsuo and K. Kangawa, Nature, 402, 656 (1999) (Original)

Ghrelin is distributed through Peptide Institute, Inc. under the license of Dr. K. Kangawa