Orexin A (Human)
(Rat, Mouse, Bovine)

4346-s 0.1 mg | 165.00 EUR

Pyr – Pro – Leu – Pro – Asp – Cys – Cys – Arg – Gln – Lys – Thr – Cys – Ser – Cys – Arg – Leu – Tyr – Glu – Leu – Leu – His – Gly – Ala – Gly – Asn – His – Ala – Ala – Gly – Ile – Leu – Thr – Leu – NH₂

Synthetic Product (Disulfide bonds between Cys⁶– Cys¹² and Cys⁷– Cys¹⁴)

The purity is guaranteed to be higher than 99% by HPLC

Appetite-Boosting Peptide

Orexin A is a 33-residue peptide with two intramolecular disulfide linkages, while Orexin B is a linear 28-residue peptide. The mutual sequence similarity is high, especially in their C-terminal regions. Human, rat and mouse peptide sequences have been determined and the primary structure of orexin-A is completely conserved among these species including bovine. When orexin-A and B are administered into the lateral hypothalamus (the brain’s “feeding center”), food intake was stimulated in a dose-dependent manner (3/30 nmol) for a few hours with slight duration difference. In addition, hypothalamic orexin mRNA is found to be up-regulated in fasting. Orexin-A and B are coded tandemly in the same mRNA flanked by the double basic amino acid residues.

The same mRNA was isolated by another group from rat hypothalamic mRNA clone; the putative reading frames corresponding to orexin-A and B are named hypocretin based on the sequence similarity to secretin. [Proc. Natl. Acad. Sci., 95, 322 (1998)]

Newly discovered appetite-boosting peptides orexin-A and B may be useful tools to analyze the regulation mechanism of feeding behavior.

Feeding behaviour is regulated by many compounds including peptides and proteins; one of the recent topics in this field is the discovery of the appetite-suppressing protein leptin. Very recently, two homologous peptides, which stimulate food consumption, have been isolated and named orexin-A and orexin-B[Cell,92, 573 (1998)]. The name orexin is from the Greek word [orexis] for appetite. These peptides were discovered in studies carried out to determine the endogenous ligands to the orphan receptor OX1R and OX2R; the notable sequence similarity of these receptors lies in the sequence of the Y2 neuropeptide Y (NPY) receptor.

Shipping | Storage | Stability
Our product Orexin A will be shipped at room temperature. Information about product specific storage conditions can be found on the vial. Stock solutions should be stored at -20°C. Please avoid repeated freezing and thawing by preparing aliquots.

References:

1. T. Sakurai, A. Amemiya, M. Ishii, I. Matsuzaki, R.M. Chemelli, H. Tanaka, S.C. Williams, J.A. Richardson, G.P. Kozlowski, S. Wilson, J.R.S. Arch, R.E. Buckingham, A.C. Haynes, S.A. Carr, R.S. Annan, D.E. McNulty, W.-S. Liu, J.A. Terrett, N.A. Elshourbagy, D.J. Bergsma and M. Yanagisawa, Cell, 92, 573 (1998) (Original)
2. L. de Lecea, T.S. Kilduff, C. Peyron, X.-B. Gao, P.E. Foye, P.E. Danielson, C. Fukuhara, E.L.F. Battenberg, V.T. Gautvik, F.S. Bartlett II, W.N. Frankel, A.N. van den Pol, F.E. Bloom, K.M. Gautvik and J.G. Sutcliffe, Proc. Natl. Acad. Sci. USA, 95, 322 (1998) (cDNA; SAme Sequence [Hypocretin])