RFamide Related Peptide-1 (Human)

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RFamide Related Peptide-1 (Human)
RFRP-1 (Human) | Prepro (56-92) Amide

4380-s 0.1 mg | 105.00 EUR

Ser – Leu – Asn – Phe – Glu – Glu – Leu – Lys – Asp – Trp – Gly – Pro – Lys – Asn – Val – Ile – Lys – Met – Ser – Thr – Pro – Ala – Val – Asn – Lys – Met – Pro – His – Ser – Phe – Ala – Asn – Leu – Pro – Leu – Arg – Phe – NH2

(M.W. 4256.9) C195H304N52O51S2

Synthetic Product

The purity is guaranteed to be higher than 99% by HPLC

Prolactin Secreting Peptide

Novel Neuropeptide with the carboxyl C-terminal Arg-Phe-NH2

Among peptides with the carboxyl (C)-terminal Arg-Phe-NH2 (RFamide family of peptides), FMRF-Amide code 4142 was first isolated from Mollusks in 1977 [Science, 197, 670 (1977)]. Another member of this family, Leu-Pro-Leu-Arg-Phe-NH2 code 4144, was identified in chicken brain in 1983, yet there had not been any reports of corresponding mammalian peptides with the same five C-terminal residues. Recently, a new related peptide was deduced from a human database search by elucidating its endogenous form through application of peptide/DNA chemistry. The name of the target peptide is RFamide-related peptide-1 (RFRP-1).

Human RFRP-1 was first predicted to be a 12 amino acid residue peptide. Subsequent synthesis and biochemical studies found it to be a ligand for the orphan receptor, OT7T022 (also called FF1) [Nat. Cell Biol., 2, 703 (2000) ]. Later, the endogenous form of human RFRP-1 was determined to be composed of 37 amino acid residues including the above 12-residue peptide at its C-terminus [Biochim. Biophys. Acta., 1540, 221 (2001) ]. The same peptide was isolated by another group who named it NPSF(1-37) [J. Biol. Chem., 276, 36961 (2001) ]. Human RFRP-1 inhibits forskolin-stimulated cAMP production in CHO cells expressing OT7T022 (ED50=21 nM). The potency is slightly less than that of the 12-residue peptide (ED50=4.9 nM), however, this result suggests that the C-terminal portion of RFRP-1 is responsible for the recognition of OT7T022. Immunoreactive RFRP-1 was observed in the central nervous system (CNS), with the highest amount detected in the hypothalamus. Specific receptors of RFRP-1, OT7T022 or FF1, have been found in the CNS. It is interesting to note that the synthetic peptide composed of 12 amino acid residues described above has shown prolactin-releasing activity at 10 nM [Nat. Cell Biol., 2, 703 (2000) ] and that rat NPSF(1-37) has the anti-opioid effect in two models of nociception at 1.0 nmol/rat [J. Biol. Chem., 276, 36961 (2001) ].

This new synthetic product RFRP-1 should serve as an important biochemical tool to further research investigations.

References:

  1. S. Hinuma, Y. Shintani, S. Fukusumi, N. Iijima, Y. Matsumoto, M. Hosoya, R. Fujii, T. Watanabe, K. Kikuchi, Y. Terao, T. Yano, T. Yamamoto, Y. Kawamata, Y. Habata, M. Asada, C. Kitada, T. Kurokawa, H. Onda, O. Nishimura, M. Tanaka, Y. Ibata and M. Fujino, Nat. Cell Biol., 2, 703 (2000) (Original; cDNA & Pharmacol.)
  2. S. Fukusumi, Y. Habata, H. Yoshida, N. Iijima, Y. Kawamata, M. Hosoya, R. Fujii, S. Hinuma, C. Kitada, Y. Shintani, M. Suenaga, H. Onda, O. Nishimura, M. Tanaka, Y. Ibata and M. Fujino, Biochim. Biophys. Acta., 1540, 221 (2001) (Endogenous Form)

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