2-NBDG

23002-v 0.5 mg | 45.50 EUR

2- [N- (7- Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino]- 2-Deoxy-D-Glucose

(M.W. 342.26)

C12H14N4O8

Synthetic Product

2-NBDG 2-[N-(7-Nitrobenz-2-Oxa- 1,3-Diazol-4-yl)Amino]- 2-Deoxy-D-Glucose is a very useful fluorescent indicator for the direct monitoring of glucose uptake into living cells. The easy to use flow cytometric assay is based on direct incubation of mammalian cells with 2-NBDG.

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References:

1. K. Yoshioka, H. Takahashi, T. Homma, M. Saito, K.-B. Oh, Y. Nemoto and H. Matsuoka, Biochim. Biophys. Acta 1289, 5 (1996) (Original; Description of 2-NBDG)
2. K. Yamada, M. Nakata, N. Horimoto, M. Saito, H. Matsuoka and N. Inagaki, J. Biol. Chem, 275, 22278 (2000) (Measurement of Glucose Uptake in Living Mammalian Cells with 2-NBDG)
3. K. Yamada, M. Saito, H. Matsuoka and N. Inagaki, Nature Protocols, 2, 753 (2007) (Chem. Synthesis of 2-NDBG & Protocols for Measurement)
4. J.V. Rocheleau, G.M. Walker, W.S. Head, O.P. McGuinness and D.W. Piston, Proc. Natl. Acad. Sci. U.S.A. 101, 12899 (2004) (Monitoring of Glucose Uptake by using 2-NBDG and NAD(P)H Response in Islet)