E-64

Irreversible inhibitor of cysteine proteases

4096-v	0.5 mg	36.00 EUR

[(2S,3S)-3-carboxyoxirane-2-carbonyl]-L-Leucyl-4-(guanidobutyl)amide
L-trans-Epoxysuccinyl-Leu-agmatine

(M.W. 357.41)

C15H27N5O5

[66701-25-5]

4096-25	25 mg	116.00 EUR
4096-100	100 mg	256.00 EUR

[(2S,3S)-3-carboxyoxirane-2-carbonyl]-L-Leucyl-4-(guanidobutyl)amide · ½H₂O

(M.W. 357.41 · 9.01)

C15H27N5O5 · ½H2O

[66701-25-5]

Synthetic Product

E-64 is a very effective irreversible inhibitor of cysteine proteases and blocks specific thiol proteases like papain, cathepsin B and L, bromelain and ficin. It reacts with the active site of the enzyme in an equimolar manner by forming a thioether bond with the sulfhydryl group. E-64 did not react with cathepsin A and D and does not affect cysteine residues in several other enzymes. The effective concentration of E-64 is around 1-10 µM. Aliquots of E-64 in DMSO are stable at +4° over some days and can be stored in a freezer at -20°C for about one month. Solutions in water or water/ethanol are possible as well.
The inhibitor E-64 offered from Peptanova is of synthetic origin manufactured by Peptide Institute Inc. under strict quality control.

References:

1. K. Hanada, M. Tamai, M. Yamagishi, S. Ohmura, J. Sawada and I. Tanaka, Agric. Biol. Chem., 42, 523 (1978) (Original)
2. K. Hanada, M. Tamai, S. Ohmura, J. Sawada, T. Seki and I. Tanaka, Agric. Biol. Chem., 42, 529 (1978) (Structure & Chem. Synthesis)
3. Y. Shoji-Kasai, M. Senshu, S. Iwashita and K. Imahori, Proc. Natl. Acad. Sci. USA, 85, 146 (1988) (Pharmacology of inhibior E-64)